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Microbial Ecogenomics and Forensic Archaeology: New Methods for Investigating Clandestine Gravesites

机译:微生物生态基因组学和法医考古学:秘密秘密墓碑调查的新方法

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摘要

In the mid-1990s, the crime scene toolkit was revolutionised by the introduction of DNA-based analyses such as the polymerase chain reaction, low copy number DNA analysis, short-tandem repeat typing, pulse-field gel electrophoresis and variable number tandem repeat. Since then, methodological advances in other disciplines, especially molecular microbial ecology, can now be adapted for cutting-edge applications in forensic contexts. Despite several studies and discussions, there is, however, currently very little evidence of these techniques’ adoption at the contemporary crime scene. Consequently, this article discusses some of the popular ‘omics’ and their current and potential exploitations in the ‘forensic ecogenomics’ of body decomposition in a crime scene. Thus, together with published supportive findings and discourse, knowledge gaps are identified. These then justify the need for more comprehensive, directed, concerted and global research towards state-of-the-art microecophysiology method application and/or adaptation for subsequent successful exploitations in this additional context of microbial forensics.
机译:在1990年代中期,犯罪现场工具包通过引入基于DNA的分析(例如聚合酶链反应,低拷贝数DNA分析,短串联重复打字,脉冲场凝胶电泳和可变数目串联重复)而发生了革命。从那时起,其他学科,尤其是分子微生物生态学的方法学进展,现在可以适应法医学领域的前沿应用。尽管进行了一些研究和讨论,但是,目前很少有证据证明这些技术在当代犯罪现场得到采用。因此,本文讨论了犯罪现场人体分解的“法医经济学”中一些流行的“组学”及其当前和潜在的利用。因此,连同已发表的支持性发现和论述,可以识别知识差距。然后,这些就证明了有必要针对最新的微生物生态学方法应用和/或适应性研究,开展更全面,更直接,更协调和全球的研究,以在这种微生物鉴证的其他背景下成功进行后续开发。

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